Hotspot

atpE-trnM-CAU

LSC · 9160-9269 · 110 bp

Back to Pericopsis

Pi 0.0818
MarkerSeek score 85.20
Primer available yes
Label atpE-trnM-CAU

Feature Metadata

label_name
atpE-trnM-CAU
feature_type
igs
region
LSC
length_bp
110
variable_sites
9
pi
0.0818
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-trnM-CAU_p1 CTCTCAGACGAGCTAGGACA TATTGCTTTCATACGGCGGG 55.0 50.0 58.0 58.4 209 bp 1.0 79.9
atpE-trnM-CAU_p2 CGAGCTAGGACACGAGTAGA TATTGCTTTCATACGGCGGG 55.0 50.0 58.1 58.4 201 bp 1.0 79.8
atpE-trnM-CAU_p3 AAGGCTGAAGGCAAAAGACA TATTGCTTTCATACGGCGGG 45.0 50.0 57.9 58.4 249 bp 1.0 79.0
atpE-trnM-CAU_p4 AGGCTGAAGGCAAAAGACAA TATTGCTTTCATACGGCGGG 45.0 50.0 57.9 58.4 248 bp 1.0 79.0
atpE-trnM-CAU_p5 TCTCAGACGAGCTAGGACAC TATTGCTTTCATACGGCGGG 55.0 50.0 58.3 58.4 208 bp 1.0 78.1