Hotspot

ycf1

SSC · 122927-128338 · 5412 bp

Back to Periandra

Pi 0.0174

MarkerSeek score 48.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5412
variable_sites: 94
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAAGGTTGCGGGTTTGATA	ACGATTTCCTCTAATTATAACACCA	50.0	32.0	58.5	56.1	189 bp	1.0	42.5
ycf1_p2	CCAAGGTTGCGGGTTTGATA	AACGATTTCCTCTAATTATAACACCA	50.0	30.8	58.5	56.7	190 bp	1.0	42.5
ycf1_p3	CCAAGGTTGCGGGTTTGATA	ACGATTTCCTCTAATTATAACACCAA	50.0	30.8	58.5	56.7	189 bp	1.0	42.5
ycf1_p4	CCAAGGTTGCGGGTTTGATA	AAAAACAAACGATTTCCTCTAA	50.0	27.3	58.5	52.5	197 bp	1.0	42.4
ycf1_p5	CCAAGGTTGCGGGTTTGATA	TGGATATAGATTATCATTCCAAGCA	50.0	32.0	58.5	55.7	226 bp	1.0	42.1