Hotspot

trnL-CAA

SSC · 113048-113128 · 81 bp

Back to Periandra

Pi 0.316

MarkerSeek score 74.90

Primer available yes

Label trnL-CAA

Feature Metadata

label_name: trnL-CAA
feature_type: tRNA
region: SSC
length_bp: 81
variable_sites: 25
pi: 0.316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA_p1	TCCTAATTGGATGGCAGTTCC	AAAAAGGCCCGAAGTAGCAA	47.6	45.0	57.7	58.0	1192-1232 bp	1.0	76.2
trnL-CAA_p2	TCCTAATTGGATGGCAGTTCC	AAAAGGCCCGAAGTAGCAAA	47.6	45.0	57.7	58.0	1191-1231 bp	1.0	76.2
trnL-CAA_p3	TCCTAATTGGATGGCAGTTCC	ATGGGATTGTAGAGCGGGTA	47.6	50.0	57.7	57.9	1240-1280 bp	1.0	75.3
trnL-CAA_p4	TCCTAATTGGATGGCAGTTCC	ACCAATGGGATTGTAGAGCG	47.6	50.0	57.7	57.7	1244-1284 bp	1.0	73.4
trnL-CAA_p5	TCCTAATTGGATGGCAGTTCC	CAATGGGATTGTAGAGCGGG	47.6	55.0	57.7	58.4	1242-1282 bp	1.0	72.8