Hotspot

rpl16

LSC · 81383-82970 · 1588 bp

Back to Periandra

Pi 0.0221

MarkerSeek score 57.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1588
variable_sites: 35
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCTCGGGTGCTAATGAAAC	AGTGGAGGGAATTGGACAGA	50.0	50.0	57.9	58.0	2730-2792 bp	1.0	80.3
rpl16_p2	GCGCAAAAGATTCGAGTTCC	AGTGGAGGGAATTGGACAGA	50.0	50.0	58.1	58.0	2670-2732 bp	1.0	80.2
rpl16_p3	ACGATTGCGCAAAAGATTCG	AGTGGAGGGAATTGGACAGA	45.0	50.0	58.1	58.0	2676-2738 bp	1.0	80.2
rpl16_p4	CGATTGCGCAAAAGATTCGA	AGTGGAGGGAATTGGACAGA	45.0	50.0	57.8	58.0	2675-2737 bp	1.0	79.9
rpl16_p5	ACCTCGGGTGCTAATGAAAC	CAAGCGGGTACAAAAGGAGT	50.0	50.0	57.9	58.1	2435-2497 bp	1.0	79.9