Hotspot

ndhA

SSC · 118648-120969 · 2322 bp

Back to Periandra

Pi 0.019
MarkerSeek score 61.90
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2322
variable_sites
44
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 ACCATAAGGAACATATCGTGA TGGGAGAAGTTGATCGTTGA 38.1 45.0 54.2 56.5 2436-2447 bp 1.0 41.8
ndhA_p2 TGAACCTAGTTACCATAAGGAACA TGGGAGAAGTTGATCGTTGA 37.5 45.0 57.1 56.5 2447-2458 bp 1.0 41.8
ndhA_p3 ACCATAAGGAACATATCGTGAA TGGGAGAAGTTGATCGTTGA 36.4 45.0 55.0 56.5 2436-2447 bp 1.0 41.8
ndhA_p4 ACCATAAGGAACATATCGTGA TGGGAGAAGTTGATCGTTGAA 38.1 42.9 54.2 57.2 2436-2447 bp 1.0 41.8
ndhA_p5 TGAACCTAGTTACCATAAGGAACA TGGGAGAAGTTGATCGTTGAA 37.5 42.9 57.1 57.2 2447-2458 bp 1.0 41.8