Hotspot

rps15-ycf1

SSC · 122530-122926 · 397 bp

Back to Periandra

Pi 0.0349

MarkerSeek score 64.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 13
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCGTTGACGTTTTCCCAA	GAACCAGTTCGCCTGTCTAG	45.0	55.0	58.0	58.0	1091-1113 bp	1.0	83.2
rps15-ycf1_p2	TCCTAACTGTTCTGGTCCGT	GAACCAGTTCGCCTGTCTAG	50.0	55.0	58.0	58.0	1550-1572 bp	1.0	82.4
rps15-ycf1_p3	AACACCATGCATTGATGGGT	GAACCAGTTCGCCTGTCTAG	45.0	55.0	58.1	58.0	1355-1377 bp	1.0	82.4
rps15-ycf1_p4	CCCAGCCATAGTAGGTGAGA	GAACCAGTTCGCCTGTCTAG	55.0	55.0	57.9	58.0	1636-1658 bp	1.0	81.8
rps15-ycf1_p5	TCCCAGCCATAGTAGGTGAG	GAACCAGTTCGCCTGTCTAG	55.0	55.0	57.9	58.0	1637-1659 bp	1.0	81.8