Hotspot

rps8-rpl14

LSC · 80477-80886 · 410 bp

Back to Periandra

Pi 0.0521

MarkerSeek score 73.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 21
pi: 0.0521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.7	57.9	465-496 bp	1.0	81.3
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.4	57.9	461-492 bp	1.0	81.0
rps8-rpl14_p3	TATGTCAGCGATGGTGTCCT	GTTTCATTAGCACCCGAGGT	50.0	50.0	58.5	57.9	464-495 bp	1.0	79.7
rps8-rpl14_p4	TTATGTCAGCGATGGTGTCC	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.7	57.6	466-497 bp	1.0	78.9
rps8-rpl14_p5	GTCAGCGATGGTGTCCTTAC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.4	57.6	462-493 bp	1.0	78.6