Hotspot

psaJ-rpl33

LSC · 66744-67244 · 501 bp

Back to Periandra

Pi 0.037

MarkerSeek score 76.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 18
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTTTCCGTGGCACCA	TCACAACCGGTACATTCCAA	50.0	45.0	58.0	57.1	667-676 bp	1.0	76.2
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	TCACAACCGGTACATTCCAA	50.0	45.0	58.0	57.1	668-677 bp	1.0	76.2
psaJ-rpl33_p3	CATATCTTTCCGTGGCACCA	GATCACAACCGGTACATTCCA	50.0	47.6	58.0	58.0	669-678 bp	1.0	75.5
psaJ-rpl33_p4	CATATCTTTCCGTGGCACCA	TGATCACAACCGGTACATTCC	50.0	47.6	58.0	58.0	670-679 bp	1.0	75.5
psaJ-rpl33_p5	ACATATCTTTCCGTGGCACC	GATCACAACCGGTACATTCCA	50.0	47.6	58.0	58.0	670-679 bp	1.0	75.5