Hotspot

trnG-UCC-trnS-GCU

LSC · 53388-54216 · 829 bp

Back to Periandra

Pi 0.0311
MarkerSeek score 67.40
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
829
variable_sites
25
pi
0.0311
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCACACTTTTACCACT CAATCCGACGCTTTAGTCCA 38.1 50.0 55.5 58.0 878-898 bp 1.0 56.2
trnG-UCC-trnS-GCU_p2 ACGAATCACACTTTTACCACT AATCCGACGCTTTAGTCCAC 38.1 50.0 55.5 58.0 877-897 bp 1.0 56.2
trnG-UCC-trnS-GCU_p3 AGAACGAATCACACTTTTACCAC CAATCCGACGCTTTAGTCCA 39.1 50.0 57.3 58.0 881-901 bp 1.0 55.1
trnG-UCC-trnS-GCU_p4 GAACGAATCACACTTTTACCACT CAATCCGACGCTTTAGTCCA 39.1 50.0 57.3 58.0 880-900 bp 1.0 55.1
trnG-UCC-trnS-GCU_p5 GAACGAATCACACTTTTACCACT AATCCGACGCTTTAGTCCAC 39.1 50.0 57.3 58.0 879-899 bp 1.0 55.1