Hotspot

trnG-GCC-psbZ

LSC · 24810-25159 · 350 bp

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Pi 0.0261

MarkerSeek score 69.90

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 9
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	AAATTAAGTAGGCAGGGGCG	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.9	58.0	542-544 bp	1.0	81.5
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	502-504 bp	1.0	80.7
trnG-GCC-psbZ_p3	AAATTAAGTAGGCAGGGGCG	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.9	57.8	545-547 bp	1.0	80.0
trnG-GCC-psbZ_p4	AAATTAAGTAGGCAGGGGCG	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.9	57.8	538-540 bp	1.0	79.9
trnG-GCC-psbZ_p5	AAATTAAGTAGGCAGGGGCG	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.9	58.3	543-545 bp	1.0	79.6