Hotspot

ndhJ-trnF-GAA

LSC · 12290-12900 · 611 bp

Back to Periandra

Pi 0.0196

MarkerSeek score 68.50

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 12
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.9	58.0	751-779 bp	1.0	80.7
ndhJ-trnF-GAA_p2	TTGGATAGGATGGCCTTTGC	GCTTCAAGAATGGTCGGGAT	50.0	50.0	57.9	58.0	766-794 bp	1.0	80.3
ndhJ-trnF-GAA_p3	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	715-743 bp	1.0	80.3
ndhJ-trnF-GAA_p4	TTGGATAGGATGGCCTTTGC	AGAATGGTCGGGATAGCTCA	50.0	50.0	57.9	57.9	760-788 bp	1.0	80.0
ndhJ-trnF-GAA_p5	TTGGATAGGATGGCCTTTGC	CTGGTAGAGCAGAGGACTGA	50.0	55.0	57.9	57.9	739-767 bp	1.0	79.6