Hotspot

trnK-UUU-rbcL

LSC · 4403-5068 · 666 bp

Back to Periandra

Pi 0.014

MarkerSeek score 66.80

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 9
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGACGAACCAATTGCTTCA	AGTCCTGAATTAGCTGCTGC	45.0	50.0	58.0	58.0	821-825 bp	1.0	83.1
trnK-UUU-rbcL_p2	TGGACGAACCAATTGCTTCA	GAGGCTAGCAAATGGAGTCC	45.0	55.0	58.0	58.0	836-840 bp	1.0	83.0
trnK-UUU-rbcL_p3	TGGACGAACCAATTGCTTCA	GCTGCTTGTGAGGTATGGAA	45.0	50.0	58.0	57.9	806-810 bp	1.0	82.5
trnK-UUU-rbcL_p4	TGGACGAACCAATTGCTTCA	GGCTAGCAAATGGAGTCCTG	45.0	55.0	58.0	58.3	834-838 bp	1.0	80.7
trnK-UUU-rbcL_p5	TGGACGAACCAATTGCTTCA	GTGAGGCTAGCAAATGGAGT	45.0	50.0	58.0	57.6	838-842 bp	1.0	80.0