Hotspot

rps7

SSC · 99771-100289 · 519 bp

Back to Pereskia

Pi 0.0424

MarkerSeek score 70.30

Primer available yes

Label rps7

Feature Metadata

label_name: rps7
feature_type: gene
region: SSC
length_bp: 519
variable_sites: 22
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	AACCGCATCCAATCTCCAAA	TTCTTGAACCCCTTTCACGC	45.0	50.0	57.8	58.7	585-795 bp	1.0	75.4
rps7_p2	ACCGCATCCAATCTCCAAAA	TTCTTGAACCCCTTTCACGC	45.0	50.0	57.8	58.7	584-794 bp	1.0	75.4
rps7_p3	GTAACCGCATCCAATCTCCA	TTCTTGAACCCCTTTCACGC	50.0	50.0	57.7	58.7	587-797 bp	1.0	74.5
rps7_p4	ACCGCATCCAATCTCCAAAA	TCTTGAACCCCTTTCACGC	45.0	52.6	57.8	58.0	583-793 bp	1.0	72.7
rps7_p5	ACCGCATCCAATCTCCAAAA	TTGAACCCCTTTCACGCTC	45.0	52.6	57.8	58.0	581-791 bp	1.0	72.7