Hotspot

rps3

LSC · 86801-87457 · 657 bp

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Pi 0.035

MarkerSeek score 68.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 23
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	AGACAAGTCAAGTAAAGGCT	ACAAGTTTGGGCTGCAGTAA	40.0	45.0	54.2	57.9	774 bp	1.0	51.4
rps3_p2	AGACAAGTCAAGTAAAGGCT	GGGCTGCAGTAAACTCTTCA	40.0	50.0	54.2	57.8	766 bp	1.0	50.5
rps3_p3	AGACAAGTCAAGTAAAGGCT	TGGGCTGCAGTAAACTCTTC	40.0	50.0	54.2	57.8	767 bp	1.0	50.5
rps3_p4	AGACAAGTCAAGTAAAGGCTT	ACAAGTTTGGGCTGCAGTAA	38.1	45.0	55.1	57.9	774 bp	1.0	50.4
rps3_p5	AAGACAAGTCAAGTAAAGGCT	ACAAGTTTGGGCTGCAGTAA	38.1	45.0	55.1	57.9	775 bp	1.0	50.3