Hotspot

accD

LSC · 60683-62212 · 1530 bp

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Pi 0.228

MarkerSeek score 82.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 318
pi: 0.228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GTTTCGACGGATCCCATTCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2136-2415 bp	1.0	91.6
accD_p2	GAAGACATGGTTTCGACGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2145-2424 bp	1.0	91.2
accD_p3	GTTTCGACGGATCCCATTCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2142-2421 bp	1.0	91.0
accD_p4	GTTTCGACGGATCCCATTCA	GGGGATTCCCGGAAAATCTC	50.0	55.0	58.0	58.0	2005 bp	0.5	59.7
accD_p5	GAAGACATGGTTTCGACGGA	GGGGATTCCCGGAAAATCTC	50.0	55.0	57.9	58.0	2014 bp	0.5	59.3