Hotspot

ycf2-trnI-CAU

IRa · 140101-140421 · 321 bp

Back to Pereskia

Pi 0.0886

MarkerSeek score 73.10

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 321
variable_sites: 28
pi: 0.0886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	940-1028 bp	1.0	83.4
ycf2-trnI-CAU_p2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	941-1029 bp	1.0	83.4
ycf2-trnI-CAU_p3	CCAGCGACGGTTTCATTAGA	TATTGGAGGGTCCCATTGGT	50.0	50.0	58.0	58.0	1003-1091 bp	1.0	83.2
ycf2-trnI-CAU_p4	TCCAGCGACGGTTTCATTAG	TATTGGAGGGTCCCATTGGT	50.0	50.0	58.0	58.0	1004-1092 bp	1.0	83.2
ycf2-trnI-CAU_p5	AATCATGGTCCTTGCGGATC	TATTGGAGGGTCCCATTGGT	50.0	50.0	58.0	58.0	1095-1183 bp	1.0	82.8