Hotspot

trnV-GAC-rrn16

SSC · 102733-102960 · 228 bp

Back to Pereskia

Pi 0.0614

MarkerSeek score 68.10

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: SSC
length_bp: 228
variable_sites: 14
pi: 0.0614
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	GTAAGATCCTTGGCGCAAGA	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	879-883 bp	1.0	81.3
trnV-GAC-rrn16_p2	ATGAATCGTCCTTTCCACGG	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	1150-1156 bp	1.0	81.2
trnV-GAC-rrn16_p3	GTAAGATCCTTGGCGCAAGA	TAACGCTTGCATCCTCTGTC	50.0	50.0	58.0	58.0	1207-1211 bp	1.0	81.0
trnV-GAC-rrn16_p4	ATGAATCGTCCTTTCCACGG	CATTGCGGAAAATTCCCCAC	50.0	50.0	58.0	58.0	1324 bp	0.5	59.7
trnV-GAC-rrn16_p5	GTAAGATCCTTGGCGCAAGA	CATTGCGGAAAATTCCCCAC	50.0	50.0	58.0	58.0	1053 bp	0.5	59.7