Hotspot

trnI-CAU-ycf2

IRb · 89792-90112 · 321 bp

Back to Pereskia

Pi 0.0886

MarkerSeek score 73.10

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 321
variable_sites: 28
pi: 0.0886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	50.0	50.0	58.0	58.0	940-1028 bp	1.0	83.4
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	50.0	50.0	58.0	58.0	941-1029 bp	1.0	83.4
trnI-CAU-ycf2_p3	TATTGGAGGGTCCCATTGGT	CCAGCGACGGTTTCATTAGA	50.0	50.0	58.0	58.0	1003-1091 bp	1.0	83.2
trnI-CAU-ycf2_p4	TATTGGAGGGTCCCATTGGT	TCCAGCGACGGTTTCATTAG	50.0	50.0	58.0	58.0	1004-1092 bp	1.0	83.2
trnI-CAU-ycf2_p5	TATTGGAGGGTCCCATTGGT	AATCATGGTCCTTGCGGATC	50.0	50.0	58.0	58.0	1095-1183 bp	1.0	82.8