Hotspot

infA-rps8

LSC · 83898-84009 · 112 bp

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Pi 0.0721

MarkerSeek score 70.50

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 8
pi: 0.0721
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ATCTAAACGAACGCGGAACA	ATCGGGAAGCTCGACTAGAA	45.0	50.0	57.9	57.9	245-246 bp	1.0	84.9
infA-rps8_p2	TCTAAACGAACGCGGAACAT	ATCGGGAAGCTCGACTAGAA	45.0	50.0	57.9	57.9	244-245 bp	1.0	84.9
infA-rps8_p3	TGCGGATCAGACGACATTTT	ATCGGGAAGCTCGACTAGAA	45.0	50.0	57.9	57.9	599-600 bp	1.0	82.0
infA-rps8_p4	GTGCACTCTTACGCGAACTA	ATCGGGAAGCTCGACTAGAA	50.0	50.0	58.0	57.9	852-854 bp	1.0	81.7
infA-rps8_p5	GTGCACTCTTACGCGAACTA	AAAAAGAGTCAGTCGGCCTG	50.0	50.0	58.0	58.1	965-967 bp	1.0	81.0