Hotspot

ATPE-trnM-CAU

LSC · 58091-58387 · 297 bp

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Pi 0.0657
MarkerSeek score 82.20
Primer available yes
Label ATPE-trnM-CAU

Feature Metadata

label_name
ATPE-trnM-CAU
feature_type
igs
region
LSC
length_bp
297
variable_sites
19
pi
0.0657
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ATPE-trnM-CAU_p1 AGACACGAGTAGAGGCTACC TATTGCTTTCATACGGCGGG 55.0 50.0 58.0 58.4 381 bp 1.0 82.0
ATPE-trnM-CAU_p2 CAGACGAGCTAAGACACGAG TATTGCTTTCATACGGCGGG 55.0 50.0 57.8 58.4 392 bp 1.0 80.9
ATPE-trnM-CAU_p3 CTCAGACGAGCTAAGACACG TATTGCTTTCATACGGCGGG 55.0 50.0 57.8 58.4 394 bp 1.0 80.9
ATPE-trnM-CAU_p4 AGACGAGCTAAGACACGAGT TATTGCTTTCATACGGCGGG 50.0 50.0 58.2 58.4 391 bp 1.0 80.7
ATPE-trnM-CAU_p5 TCAGACGAGCTAAGACACGA TATTGCTTTCATACGGCGGG 50.0 50.0 58.2 58.4 393 bp 1.0 80.6