primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ATPE-trnM-CAU_p1	ATPE-trnM-CAU	1	AGACACGAGTAGAGGCTACC	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	58.0	58.4	0.0	13.2	0.452577	58091	58387	381-381	1.000000	82.0
ATPE-trnM-CAU_p2	ATPE-trnM-CAU	2	CAGACGAGCTAAGACACGAG	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	57.8	58.4	0.0	13.2	0.578067	58091	58387	392-392	1.000000	80.9
ATPE-trnM-CAU_p3	ATPE-trnM-CAU	3	CTCAGACGAGCTAAGACACG	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	57.8	58.4	0.0	13.2	0.578067	58091	58387	394-394	1.000000	80.9
ATPE-trnM-CAU_p4	ATPE-trnM-CAU	4	AGACGAGCTAAGACACGAGT	TATTGCTTTCATACGGCGGG	20	20	50.0	50.0	58.2	58.4	0.0	13.2	0.600367	58091	58387	391-391	1.000000	80.7
ATPE-trnM-CAU_p5	ATPE-trnM-CAU	5	TCAGACGAGCTAAGACACGA	TATTGCTTTCATACGGCGGG	20	20	50.0	50.0	58.2	58.4	0.0	13.2	0.606725	58091	58387	393-393	1.000000	80.6
trnV-UAC-accD_p1	trnV-UAC-accD	1	CCGATTCAGGTGCTCGTTTA	CCACCGATCCCATACTACCT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.015705	59286	60682	2962-2962	0.500000	59.9
trnV-UAC-accD_p2	trnV-UAC-accD	2	CCGATTCAGGTGCTCGTTTA	CCTACCACCGATCCCATACT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.015705	59286	60682	2966-2966	0.500000	59.9
trnV-UAC-accD_p3	trnV-UAC-accD	3	CCGATTCAGGTGCTCGTTTA	GCGGAATTTTGAGCAAGACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021970	59286	60682	2687-2687	0.500000	59.8
trnV-UAC-accD_p4	trnV-UAC-accD	4	CCGATTCAGGTGCTCGTTTA	GGCGGAATTTTGAGCAAGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021970	59286	60682	2688-2688	0.500000	59.8
trnV-UAC-accD_p5	trnV-UAC-accD	5	CCGATTCAGGTGCTCGTTTA	GCGGCATATTCAATCAACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041139	59286	60682	2999-2999	0.500000	59.7
accD_p1	accD	1	GTTTCGACGGATCCCATTCA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	6.1	26.9	0.045545	60683	62212	2136-2415	1.000000	91.6
accD_p2	accD	2	GAAGACATGGTTTCGACGGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.101550	60683	62212	2145-2424	1.000000	91.2
accD_p3	accD	3	GTTTCGACGGATCCCATTCA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	6.1	26.8	0.122079	60683	62212	2142-2421	1.000000	91.0
accD_p4	accD	4	GTTTCGACGGATCCCATTCA	GGGGATTCCCGGAAAATCTC	20	20	50.0	55.0	58.0	58.0	6.1	21.5	0.035882	60683	62212	2005-2005	0.500000	59.7
accD_p5	accD	5	GAAGACATGGTTTCGACGGA	GGGGATTCCCGGAAAATCTC	20	20	50.0	55.0	57.9	58.0	0.0	21.5	0.091887	60683	62212	2014-2014	0.500000	59.3
rpl33-rps18_p1	rpl33-rps18	1	TCTGTCCCTATTGTTACAGACA	AAGGGTCGCTTGGATTTATC	22	20	40.9	45.0	56.2	55.5	0.0	0.6	6.278233	71849	72177	402-407	1.000000	46.5
rpl33-rps18_p2	rpl33-rps18	2	TCTGTCCCTATTGTTACAGACA	AAAGGGTCGCTTGGATTTATC	22	21	40.9	42.9	56.2	56.3	0.0	2.5	6.486244	71849	72177	403-408	1.000000	46.5
rpl33-rps18_p3	rpl33-rps18	3	TTCTGTCCCTATTGTTACAGACA	AAGGGTCGCTTGGATTTATC	23	20	39.1	45.0	56.9	55.5	3.6	0.6	6.571767	71849	72177	403-408	1.000000	46.5
rpl33-rps18_p4	rpl33-rps18	4	TCTGTCCCTATTGTTACAGACAT	AAGGGTCGCTTGGATTTATC	23	20	39.1	45.0	56.7	55.5	5.1	0.6	6.775275	71849	72177	402-407	1.000000	46.5
rpl33-rps18_p5	rpl33-rps18	5	TTCTGTCCCTATTGTTACAGACA	AAAGGGTCGCTTGGATTTATC	23	21	39.1	42.9	56.9	56.3	3.6	2.5	6.779778	71849	72177	404-409	1.000000	46.5
infA-rps8_p1	infA-rps8	1	ATCTAAACGAACGCGGAACA	ATCGGGAAGCTCGACTAGAA	20	20	45.0	50.0	57.9	57.9	12.5	0.0	0.123069	83898	84009	245-246	1.000000	84.9
infA-rps8_p2	infA-rps8	2	TCTAAACGAACGCGGAACAT	ATCGGGAAGCTCGACTAGAA	20	20	45.0	50.0	57.9	57.9	12.5	0.0	0.123069	83898	84009	244-245	1.000000	84.9
infA-rps8_p3	infA-rps8	3	TGCGGATCAGACGACATTTT	ATCGGGAAGCTCGACTAGAA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.147538	83898	84009	599-600	1.000000	82.0
infA-rps8_p4	infA-rps8	4	GTGCACTCTTACGCGAACTA	ATCGGGAAGCTCGACTAGAA	20	20	50.0	50.0	58.0	57.9	15.9	0.0	0.081094	83898	84009	852-854	1.000000	81.7
infA-rps8_p5	infA-rps8	5	GTGCACTCTTACGCGAACTA	AAAAAGAGTCAGTCGGCCTG	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.142627	83898	84009	965-967	1.000000	81.0
rps3_p1	rps3	1	AGACAAGTCAAGTAAAGGCT	ACAAGTTTGGGCTGCAGTAA	20	20	40.0	45.0	54.2	57.9	2.7	16.6	3.867902	86801	87457	774-774	1.000000	51.4
rps3_p2	rps3	2	AGACAAGTCAAGTAAAGGCT	GGGCTGCAGTAAACTCTTCA	20	20	40.0	50.0	54.2	57.8	2.7	16.6	3.987008	86801	87457	766-766	1.000000	50.5
rps3_p3	rps3	3	AGACAAGTCAAGTAAAGGCT	TGGGCTGCAGTAAACTCTTC	20	20	40.0	50.0	54.2	57.8	2.7	16.6	3.987008	86801	87457	767-767	1.000000	50.5
rps3_p4	rps3	4	AGACAAGTCAAGTAAAGGCTT	ACAAGTTTGGGCTGCAGTAA	21	20	38.1	45.0	55.1	57.9	0.0	16.6	4.003145	86801	87457	774-774	1.000000	50.4
rps3_p5	rps3	5	AAGACAAGTCAAGTAAAGGCT	ACAAGTTTGGGCTGCAGTAA	21	20	38.1	45.0	55.1	57.9	2.7	16.6	4.003145	86801	87457	775-775	1.000000	50.3
rps19-rpl2_p1	rps19-rpl2	1	AAATGGGCAAGTGTTCCCTT	ATCCCAAAACTGCTCAGCAA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.101991	88334	88390	518-524	1.000000	80.6
rps19-rpl2_p2	rps19-rpl2	2	AGTCGGTACAAACTCTCCCA	ATCCCAAAACTGCTCAGCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.005722	88334	88390	568-568	0.500000	60.0
rps19-rpl2_p3	rps19-rpl2	3	AGTCGGTACAAACTCTCCCA	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.008572	88334	88390	506-506	0.500000	59.9
rps19-rpl2_p4	rps19-rpl2	4	AGTCGGTACAAACTCTCCCA	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	22.9	0.059790	88334	88390	407-407	0.500000	59.5
rps19-rpl2_p5	rps19-rpl2	5	AGTCGGTACAAACTCTCCCA	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.099733	88334	88390	513-513	0.500000	59.2
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	89792	90112	940-1028	1.000000	83.4
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	89792	90112	941-1029	1.000000	83.4
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TATTGGAGGGTCCCATTGGT	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	11.6	0.0	0.010184	89792	90112	1003-1091	1.000000	83.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	TATTGGAGGGTCCCATTGGT	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	11.6	0.0	0.010184	89792	90112	1004-1092	1.000000	83.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TATTGGAGGGTCCCATTGGT	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.0	58.0	11.6	0.0	0.032729	89792	90112	1095-1183	1.000000	82.8
rps7_p1	rps7	1	AACCGCATCCAATCTCCAAA	TTCTTGAACCCCTTTCACGC	20	20	45.0	50.0	57.8	58.7	0.0	0.0	0.899646	99771	100289	585-795	1.000000	75.4
rps7_p2	rps7	2	ACCGCATCCAATCTCCAAAA	TTCTTGAACCCCTTTCACGC	20	20	45.0	50.0	57.8	58.7	0.0	0.0	0.899646	99771	100289	584-794	1.000000	75.4
rps7_p3	rps7	3	GTAACCGCATCCAATCTCCA	TTCTTGAACCCCTTTCACGC	20	20	50.0	50.0	57.7	58.7	0.0	0.0	1.010853	99771	100289	587-797	1.000000	74.5
rps7_p4	rps7	4	ACCGCATCCAATCTCCAAAA	TCTTGAACCCCTTTCACGC	20	19	45.0	52.6	57.8	58.0	0.0	0.0	1.229892	99771	100289	583-793	1.000000	72.7
rps7_p5	rps7	5	ACCGCATCCAATCTCCAAAA	TTGAACCCCTTTCACGCTC	20	19	45.0	52.6	57.8	58.0	0.0	0.0	1.229892	99771	100289	581-791	1.000000	72.7
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	GTAAGATCCTTGGCGCAAGA	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	10.8	0.0	0.029546	102733	102960	879-883	1.000000	81.3
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	ATGAATCGTCCTTTCCACGG	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.029546	102733	102960	1150-1156	1.000000	81.2
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	GTAAGATCCTTGGCGCAAGA	TAACGCTTGCATCCTCTGTC	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.034321	102733	102960	1207-1211	1.000000	81.0
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	ATGAATCGTCCTTTCCACGG	CATTGCGGAAAATTCCCCAC	20	20	50.0	50.0	58.0	58.0	0.0	6.6	0.032927	102733	102960	1324-1324	0.500000	59.7
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	GTAAGATCCTTGGCGCAAGA	CATTGCGGAAAATTCCCCAC	20	20	50.0	50.0	58.0	58.0	10.8	6.6	0.032927	102733	102960	1053-1053	0.500000	59.7
ndhF_p1	ndhF	1	GAGCTACTGAGGAACAACGG	AAGAACCGGCGAATCGTAAA	20	20	55.0	45.0	58.0	57.9	0.0	9.3	0.085497	111621	113864	2646-2646	0.500000	59.3
ndhF_p2	ndhF	2	CCCGGAACTTCTTCGTAGTG	AAGAACCGGCGAATCGTAAA	20	20	55.0	45.0	58.0	57.9	0.7	9.3	0.090997	111621	113864	2551-2551	0.500000	59.3
ndhF_p3	ndhF	3	CCGGAACTTCTTCGTAGTGG	AAGAACCGGCGAATCGTAAA	20	20	55.0	45.0	58.0	57.9	0.0	9.3	0.090997	111621	113864	2550-2550	0.500000	59.3
ndhF_p4	ndhF	4	ACTCCCGGAACTTCTTCGTA	AAGAACCGGCGAATCGTAAA	20	20	50.0	45.0	58.1	57.9	0.7	9.3	0.168026	111621	113864	2554-2554	0.500000	58.7
ndhF_p5	ndhF	5	TCCCGGAACTTCTTCGTAGT	AAGAACCGGCGAATCGTAAA	20	20	50.0	45.0	58.1	57.9	0.7	9.3	0.168026	111621	113864	2552-2552	0.500000	58.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGTCGTCGGAAAAAGGA	TTAACGCTGCCCAATATCCC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.337063	113865	115099	1422-1422	0.500000	57.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGTCGTCGGAAAAAGGA	AAAAGCTTTTAACGCTGCCC	20	20	45.0	45.0	57.7	57.9	0.0	22.7	0.461638	113865	115099	1430-1430	0.500000	56.3
ndhF-rpl32_p3	ndhF-rpl32	3	CGGAAAAAGGAGAAGTCCCA	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.585818	113865	115099	1413-1413	0.500000	55.3
ndhF-rpl32_p4	ndhF-rpl32	4	CGGAAAAAGGAGAAGTCCCA	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	57.4	57.9	0.0	22.7	0.710394	113865	115099	1421-1421	0.500000	54.3
ndhF-rpl32_p5	ndhF-rpl32	5	TCGGAAAAAGGAGAAGTCCC	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.877575	113865	115099	1414-1414	0.500000	53.0
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	140101	140421	940-1028	1.000000	83.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	140101	140421	941-1029	1.000000	83.4
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CCAGCGACGGTTTCATTAGA	TATTGGAGGGTCCCATTGGT	20	20	50.0	50.0	58.0	58.0	0.0	11.6	0.010184	140101	140421	1003-1091	1.000000	83.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TCCAGCGACGGTTTCATTAG	TATTGGAGGGTCCCATTGGT	20	20	50.0	50.0	58.0	58.0	0.0	11.6	0.010184	140101	140421	1004-1092	1.000000	83.2
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	AATCATGGTCCTTGCGGATC	TATTGGAGGGTCCCATTGGT	20	20	50.0	50.0	58.0	58.0	0.0	11.6	0.032729	140101	140421	1095-1183	1.000000	82.8