Hotspot

rpl23

IRa · 153979-154260 · 282 bp

Back to Peperomia

Pi NA

MarkerSeek score 31.40

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: IRa
length_bp: 282
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	TCGATACTGGAAATTATAGGGA	GACTGTCTACGGCTCCTTTG	36.4	55.0	53.6	58.0	396 bp	0.5	20.0
rpl23_p2	TCGATACTGGAAATTATAGGGA	CACTTGACTGTCTACGGCTC	36.4	55.0	53.6	58.0	401 bp	0.5	20.0
rpl23_p3	TCGATACTGGAAATTATAGGGAA	GACTGTCTACGGCTCCTTTG	34.8	55.0	54.4	58.0	396 bp	0.5	20.0
rpl23_p4	TCGATACTGGAAATTATAGGGAA	CACTTGACTGTCTACGGCTC	34.8	55.0	54.4	58.0	401 bp	0.5	20.0
rpl23_p5	TCGATACTGGAAATTATAGGGA	TGACTGTCTACGGCTCCTTT	36.4	50.0	53.6	58.4	397 bp	0.5	20.0