Hotspot

ndhD-psaC

SSC · 120827-120966 · 140 bp

Back to Peperomia

Pi 0.0677

MarkerSeek score 75.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 140
variable_sites: 9
pi: 0.0677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGAATAAACCCGCAAATATCGG	CGAAACAACTCGCAGTATGG	40.9	50.0	57.2	57.2	211-235 bp	1.0	58.4
ndhD-psaC_p2	TGAATAAACCCGCAAATATCGG	CACGAAACAACTCGCAGTATG	40.9	47.6	57.2	57.9	213-237 bp	1.0	55.9
ndhD-psaC_p3	TGAATAAACCCGCAAATATCGG	GCACGAAACAACTCGCAGTA	40.9	50.0	57.2	59.1	214-238 bp	1.0	55.5
ndhD-psaC_p4	ATGAATAAACCCGCAAATATCGG	CGAAACAACTCGCAGTATGG	39.1	50.0	57.6	57.2	212-236 bp	1.0	53.9
ndhD-psaC_p5	CGCAAATATCGGAAAAACAACA	CGAAACAACTCGCAGTATGG	36.4	50.0	56.5	57.2	201-225 bp	1.0	53.3