Hotspot

rpl14-rpl16

LSC · 84163-84299 · 137 bp

Back to Peperomia

Pi 0.118

MarkerSeek score 74.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 16
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGTGTCCCTACCCATGACAA	GTCGCGATTTCAATAGCTGC	50.0	50.0	58.0	58.0	818 bp	1.0	83.3
rpl14-rpl16_p2	AGTGTCCCTACCCATGACAA	GAGGTCGGGGGAATATCAGA	50.0	55.0	58.0	58.0	851 bp	1.0	83.3
rpl14-rpl16_p3	AGTGTCCCTACCCATGACAA	GCGATTTCAATAGCTGCGTC	50.0	50.0	58.0	58.0	815 bp	1.0	83.2
rpl14-rpl16_p4	AGTGTCCCTACCCATGACAA	CCCGTTACAGTAAGACCTGC	50.0	55.0	58.0	58.0	947 bp	0.5	59.6
rpl14-rpl16_p5	AGTGTCCCTACCCATGACAA	GCCCGTTACAGTAAGACCTG	50.0	55.0	58.0	58.0	948 bp	0.5	59.6