Hotspot

psaJ-rpl33

LSC · 69956-70383 · 428 bp

Back to Peperomia

Pi 0.0598

MarkerSeek score 72.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 25
pi: 0.0598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCTACACCCCTTTCCATCAG	AATCGACCGGGTGTATTGTG	55.0	50.0	58.0	58.0	1186-1231 bp	1.0	85.1
psaJ-rpl33_p2	GCTACACCCCTTTCCATCAG	CCGGGTGTATTGTGTCGATT	55.0	50.0	58.0	58.0	1180-1225 bp	1.0	85.1
psaJ-rpl33_p3	AGGCTTTGCAATGCGAGATA	AATCGACCGGGTGTATTGTG	45.0	50.0	58.0	58.0	717-746 bp	1.0	84.0
psaJ-rpl33_p4	AGGCTTTGCAATGCGAGATA	CCGGGTGTATTGTGTCGATT	45.0	50.0	58.0	58.0	711-740 bp	1.0	84.0
psaJ-rpl33_p5	AGGCTTTGCAATGCGAGATA	TATCTTGAAACGCCCGATGG	45.0	50.0	58.0	58.1	678-707 bp	1.0	84.0