Hotspot

petG-trnW-CCA

LSC · 68883-69024 · 142 bp

Back to Peperomia

Pi 0.0942

MarkerSeek score 80.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 13
pi: 0.0942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGGCGATCAGTTAGACCTTT	TTCAAATCCTACAGGGCGTG	50.0	50.0	57.7	57.9	180-184 bp	1.0	84.2
petG-trnW-CCA_p2	CGGCGATCAGTTAGACCTTT	GTTCAAATCCTACAGGGCGT	50.0	50.0	57.7	57.9	181-185 bp	1.0	84.2
petG-trnW-CCA_p3	CGGCGATCAGTTAGACCTTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.7	58.0	235-239 bp	1.0	83.2
petG-trnW-CCA_p4	ACTTTGGCCGGATTATTCGT	TTCAAATCCTACAGGGCGTG	45.0	50.0	57.6	57.9	224-228 bp	1.0	81.8
petG-trnW-CCA_p5	ACTTTGGCCGGATTATTCGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	57.6	58.0	279-283 bp	1.0	81.4