Hotspot

ndhC-trnV-UAC

LSC · 52266-53721 · 1456 bp

Back to Peperomia

Pi 0.0341

MarkerSeek score 82.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1456
variable_sites: 47
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCCGGAAATTTGAAATGCC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1535-1577 bp	1.0	84.7
ndhC-trnV-UAC_p2	CCCCGGAAATTTGAAATGCC	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	1534-1576 bp	1.0	81.9
ndhC-trnV-UAC_p3	GGGCTAAAACCCCGGAAATT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1544-1586 bp	1.0	81.3
ndhC-trnV-UAC_p4	GGGGATTCATTAATCGGGGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.5	57.8	1560-1602 bp	1.0	81.1
ndhC-trnV-UAC_p5	CCCCGGAAATTTGAAATGCC	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.0	58.7	1523-1565 bp	1.0	80.8