Hotspot

trnT-GGU-psbD

LSC · 32121-33785 · 1665 bp

Back to Peperomia

Pi 0.0382

MarkerSeek score 73.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1665
variable_sites: 62
pi: 0.0382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTAACGCCATGGTAAGACGT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.0	58.2	1810-1853 bp	1.0	82.5
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1818-1861 bp	1.0	82.0
trnT-GGU-psbD_p3	GTAACGCCATGGTAAGACGT	CCCTTCGTAACCAGTCATCC	50.0	55.0	58.0	57.7	1794-1837 bp	1.0	82.0
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1821-1864 bp	1.0	81.8
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	55.0	55.0	58.1	57.7	1802-1845 bp	1.0	81.5