Hotspot

petN-psbM

LSC · 28863-29777 · 915 bp

Back to Peperomia

Pi 0.0462

MarkerSeek score 78.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 34
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	GCTGTTTATTCTAGTTCCTACTGC	45.0	41.7	58.0	57.4	924-1076 bp	1.0	49.9
petN-psbM_p2	ACCTTTTCCCTTTCGCTTGT	GCTGTTTATTCTAGTTCCTACTGC	45.0	41.7	57.9	57.4	893-1045 bp	1.0	49.5
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	GCTGTTTATTCTAGTTCCTACTGC	50.0	41.7	57.8	57.4	930-1082 bp	1.0	48.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTGTTTATTCTAGTTCCTACTGC	50.0	41.7	57.6	57.4	870-1022 bp	1.0	47.3
petN-psbM_p5	GGGGACATAATTGGCATGGA	GCTGTTTATTCTAGTTCCTACTGC	50.0	41.7	57.6	57.4	953-1105 bp	1.0	46.9