Hotspot

ycf1

Genome · 119920-125001 · 5082 bp

Back to Pentarhizidium

Pi 0.027

MarkerSeek score 54.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5082
variable_sites: 137
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAAAATCTGTAACTAGCCACG	ATTTCCACCCGACAAGATGC	45.5	50.0	58.0	58.5	176 bp	1.0	63.8
ycf1_p2	GCAAAATCTGTAACTAGCCACG	ACGAGATGGAGATGTGCAAA	45.5	45.0	58.0	57.2	198 bp	1.0	61.5
ycf1_p3	GCAAAATCTGTAACTAGCCACG	GTACGAGATGGAGATGTGCA	45.5	50.0	58.0	57.1	200 bp	1.0	60.6
ycf1_p4	GCAAAATCTGTAACTAGCCACG	TGTACGAGATGGAGATGTGC	45.5	50.0	58.0	57.1	201 bp	1.0	60.6
ycf1_p5	GCAAAATCTGTAACTAGCCACG	ACGAGATGGAGATGTGCAAAA	45.5	42.9	58.0	57.9	198 bp	1.0	59.1