Hotspot

ndhB

Genome · 500-1237 · 738 bp

Back to Pentarhizidium

Pi 0.0298

MarkerSeek score 61.60

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 738
variable_sites: 22
pi: 0.0298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ACGTACGCTACTGTTCGAAC	AAATCCGTGTGGCAGAAAGT	50.0	45.0	58.0	58.0	968 bp	1.0	82.5
ndhB_p2	ACGTACGCTACTGTTCGAAC	AATCCGTGTGGCAGAAAGTT	50.0	45.0	58.0	58.0	967 bp	1.0	82.5
ndhB_p3	CACGTACGCTACTGTTCGAA	AATCCGTGTGGCAGAAAGTT	50.0	45.0	58.0	58.0	968 bp	1.0	82.5
ndhB_p4	CGTACGCTACTGTTCGAACA	AAATCCGTGTGGCAGAAAGT	50.0	45.0	58.0	58.0	967 bp	1.0	82.4
ndhB_p5	CGTACGCTACTGTTCGAACA	AATCCGTGTGGCAGAAAGTT	50.0	45.0	58.0	58.0	966 bp	1.0	82.4