Hotspot

ccsA-ndhD

Genome · 111557-111966 · 410 bp

Back to Pentarhizidium

Pi 0.0569

MarkerSeek score 71.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 410
variable_sites: 23
pi: 0.0569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTATGCTGACTCACGCCTTT	TTTGGTCCGAGGGAGGTATT	50.0	50.0	58.0	58.0	1170-1177 bp	1.0	82.9
ccsA-ndhD_p2	CTATGCTGACTCACGCCTTT	TACTTGCTACGACAGAACGC	50.0	50.0	58.0	58.0	1489-1496 bp	1.0	82.6
ccsA-ndhD_p3	CTATGCTGACTCACGCCTTT	AACGGAGCCATATCGCAAAT	50.0	45.0	58.0	58.0	1554-1561 bp	1.0	82.4
ccsA-ndhD_p4	GGTTTGCTTCTTGGGAGTCA	TACTTGCTACGACAGAACGC	50.0	50.0	58.0	58.0	931 bp	0.5	59.6
ccsA-ndhD_p5	GGTTTGCTTCTTGGGAGTCA	AACGGAGCCATATCGCAAAT	50.0	45.0	58.0	58.0	996 bp	0.5	59.6