Hotspot

rpl32-trnP-GGG

Genome · 109276-109911 · 636 bp

Back to Pentarhizidium

Pi 0.0665

MarkerSeek score 76.20

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 636
variable_sites: 42
pi: 0.0665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	ATGACGGCGAGAAAAGACTC	GTACAAACGCGAGGAAGGAT	50.0	50.0	58.0	58.0	1276 bp	0.5	59.9
rpl32-trnP-GGG_p2	AATAACGCAGCTGTCAGAGG	GTACAAACGCGAGGAAGGAT	50.0	50.0	58.0	58.0	1771 bp	0.5	59.8
rpl32-trnP-GGG_p3	ATGACGGCGAGAAAAGACTC	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.0	58.0	1047 bp	0.5	59.8
rpl32-trnP-GGG_p4	ATGACGGCGAGAAAAGACTC	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1045 bp	0.5	59.8
rpl32-trnP-GGG_p5	AATAACGCAGCTGTCAGAGG	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.0	1540 bp	0.5	59.7