Hotspot

rps3-rpl22

Genome · 79518-79629 · 112 bp

Back to Pentarhizidium

Pi 0.0943

MarkerSeek score 76.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: Genome
length_bp: 112
variable_sites: 10
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAACCCGAATGAGATCCGTT	AAAATATCCGCGTGTCAGCT	50.0	45.0	58.0	58.0	662-667 bp	1.0	82.7
rps3-rpl22_p2	ACCGGTGGGAATAATGCTTC	GGTCACAAACTGGGGGAATT	50.0	50.0	57.9	58.0	647-652 bp	1.0	82.7
rps3-rpl22_p3	GAACCGGTGGGAATAATGCT	GGTCACAAACTGGGGGAATT	50.0	50.0	57.9	58.0	649-654 bp	1.0	82.7
rps3-rpl22_p4	CAACCCGAATGAGATCCGTT	GGTCACAAACTGGGGGAATT	50.0	50.0	58.0	58.0	803-808 bp	1.0	82.5
rps3-rpl22_p5	TTTCGATATGCCCAACTCCG	GGTCACAAACTGGGGGAATT	50.0	50.0	58.1	58.0	773-778 bp	1.0	82.4