Hotspot

clpP-psbB

Genome · 68008-68458 · 451 bp

Back to Pentarhizidium

Pi 0.0315

MarkerSeek score 75.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 451
variable_sites: 14
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTATACGTCAACCCGAGCTG	GTATGAACGCGATACCGAGG	55.0	55.0	57.9	58.3	546-552 bp	1.0	79.6
clpP-psbB_p2	CTATACGTCAACCCGAGCTG	GGTATGAACGCGATACCGAG	55.0	55.0	57.9	58.3	547-553 bp	1.0	79.5
clpP-psbB_p3	CACTATACGTCAACCCGAGC	GGTATGAACGCGATACCGAG	55.0	55.0	58.2	58.3	549-555 bp	1.0	79.2
clpP-psbB_p4	CACTATACGTCAACCCGAGC	GTATGAACGCGATACCGAGG	55.0	55.0	58.2	58.3	548-554 bp	1.0	79.2
clpP-psbB_p5	CTATACGTCAACCCGAGCTG	AGATGCACGGAAATCAGACG	55.0	50.0	57.9	58.4	588-594 bp	1.0	79.0