Hotspot

trnW-CCA-trnP-UGG

Genome · 62753-62927 · 175 bp

Back to Pentarhizidium

Pi 0.08
MarkerSeek score 76.40
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
Genome
length_bp
175
variable_sites
14
pi
0.08
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 ACCTACGACATCGGGTTTTG GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.9 57.9 286 bp 1.0 82.2
trnW-CCA-trnP-UGG_p2 ACCTACGACATCGGGTTTTG CGGTAGCGTGTTTGTTTTGG 50.0 50.0 57.9 58.3 287 bp 1.0 81.2
trnW-CCA-trnP-UGG_p3 CTACGACATCGGGTTTTGGA GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.6 57.9 284 bp 1.0 79.9
trnW-CCA-trnP-UGG_p4 TACGACATCGGGTTTTGGAG GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.6 57.9 283 bp 1.0 79.9
trnW-CCA-trnP-UGG_p5 GAACCTACGACATCGGGTTT GGTAGCGTGTTTGTTTTGGG 50.0 50.0 57.6 57.9 288 bp 1.0 79.9