Hotspot

trnV-UAC-trnM-CAU

Genome · 49568-49799 · 232 bp

Back to Pentarhizidium

Pi 0.0439

MarkerSeek score 76.20

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: Genome
length_bp: 232
variable_sites: 10
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	AATCACCGGGTTTCGACAAA	TTCGAACCAATGACTCTCGC	45.0	50.0	58.0	58.3	374-381 bp	1.0	81.0
trnV-UAC-trnM-CAU_p2	ATCACCGGGTTTCGACAAAT	TTCGAACCAATGACTCTCGC	45.0	50.0	57.8	58.3	373-380 bp	1.0	79.7
trnV-UAC-trnM-CAU_p3	AATCACCGGGTTTCGACAAA	CAATGACTCTCGCCGTATGA	45.0	50.0	58.0	57.5	367-374 bp	1.0	79.5
trnV-UAC-trnM-CAU_p4	AATCACCGGGTTTCGACAAA	CCAATGACTCTCGCCGTATG	45.0	55.0	58.0	58.5	368-375 bp	1.0	79.4
trnV-UAC-trnM-CAU_p5	TGATCATGCGGAGTTTTGCT	TTCGAACCAATGACTCTCGC	45.0	50.0	58.2	58.3	408 bp	0.5	56.3