Hotspot

trnS-GGA-rps4

Genome · 43631-44100 · 470 bp

Back to Pentarhizidium

Pi 0.0452
MarkerSeek score 76.50
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
Genome
length_bp
470
variable_sites
21
pi
0.0452
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 TTCGAATCCCTCCCTTTCCT AGGATTGGTGAATCGTGTCG 50.0 50.0 58.0 58.0 581-582 bp 1.0 83.2
trnS-GGA-rps4_p2 GGTTCGAATCCCTCCCTTTC AGGATTGGTGAATCGTGTCG 55.0 50.0 58.0 58.0 583-584 bp 1.0 83.2
trnS-GGA-rps4_p3 GTTCGAATCCCTCCCTTTCC AGGATTGGTGAATCGTGTCG 55.0 50.0 58.0 58.0 582-583 bp 1.0 83.2
trnS-GGA-rps4_p4 TTCGAATCCCTCCCTTTCCT GCCAATCGAGAATCCGTCAA 50.0 50.0 58.0 58.3 562-563 bp 1.0 80.6
trnS-GGA-rps4_p5 GTTCGAATCCCTCCCTTTCC GCCAATCGAGAATCCGTCAA 55.0 50.0 58.0 58.3 563-564 bp 1.0 80.6