Hotspot

psbM-petN

Genome · 28284-29151 · 868 bp

Back to Pentarhizidium

Pi 0.044

MarkerSeek score 75.40

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 868
variable_sites: 38
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CGGCCGCTCAGAATAACTAG	CCACTTGTAGTTCGGGGAAG	55.0	55.0	58.2	57.9	921-930 bp	1.0	81.0
psbM-petN_p2	CTTTACATTCAAACGGCCGC	CCACTTGTAGTTCGGGGAAG	50.0	55.0	58.4	57.9	934-943 bp	1.0	79.7
psbM-petN_p3	TCCCAATCCCAACAGCTTTT	CCACTTGTAGTTCGGGGAAG	45.0	55.0	57.6	57.9	963-972 bp	1.0	79.5
psbM-petN_p4	CCACCGCACTTTTTATCCCA	CCACTTGTAGTTCGGGGAAG	50.0	55.0	58.5	57.9	978-987 bp	1.0	79.0
psbM-petN_p5	CGGCCGCTCAGAATAACTAG	GCCCTGACGGTGATTTCTAC	55.0	55.0	58.2	58.3	951-960 bp	1.0	78.9