Hotspot

trnD-GUC-trnY-GUA

Genome · 27389-27440 · 52 bp

Back to Pentarhizidium

Pi 0.0769

MarkerSeek score 75.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 52
variable_sites: 4
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TCAATTGGTTAGAGCACCGC	TAACTCGATGAGGCGAAACC	50.0	50.0	58.6	57.7	235-240 bp	1.0	75.0
trnD-GUC-trnY-GUA_p2	TCAATTGGTTAGAGCACCGC	CTCGAGTGGTTAATGGGGAC	50.0	55.0	58.6	57.7	188 bp	1.0	74.8
trnD-GUC-trnY-GUA_p3	TCAATTGGTTAGAGCACCGC	ATGCTCGAGTGGTTAATGGG	50.0	50.0	58.6	57.7	191 bp	1.0	74.6
trnD-GUC-trnY-GUA_p4	TCAATTGGTTAGAGCACCGC	GACTGTAAATCCGCTGGCAA	50.0	50.0	58.6	58.6	167 bp	1.0	73.1
trnD-GUC-trnY-GUA_p5	GTTCGAGACCCGTCAATCC	TAACTCGATGAGGCGAAACC	57.9	50.0	58.0	57.7	193-198 bp	1.0	71.4