Hotspot

rpoB-trnD-GUC

Genome · 26553-27314 · 762 bp

Back to Pentarhizidium

Pi 0.045

MarkerSeek score 74.90

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 762
variable_sites: 34
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	TTGTGCAAGTTCTGGCAGTA	CGTTGGAGTTTACCGGGATT	45.0	50.0	57.7	57.9	875-876 bp	1.0	80.2
rpoB-trnD-GUC_p2	TGTGCAAGTTCTGGCAGTAC	CGTTGGAGTTTACCGGGATT	50.0	50.0	58.4	57.9	874-875 bp	1.0	79.7
rpoB-trnD-GUC_p3	TTTGTGCAAGTTCTGGCAGT	CGTTGGAGTTTACCGGGATT	45.0	50.0	58.5	57.9	876-877 bp	1.0	78.7
rpoB-trnD-GUC_p4	TTGTGCAAGTTCTGGCAGTA	GGAGTTTACCGGGATTGACG	45.0	55.0	57.7	58.4	871-872 bp	1.0	78.2
rpoB-trnD-GUC_p5	TGTGCAAGTTCTGGCAGTAC	GGAGTTTACCGGGATTGACG	50.0	55.0	58.4	58.4	870-871 bp	1.0	77.7