Hotspot

atpH-atpI

Genome · 13559-14325 · 767 bp

Back to Pentarhizidium

Pi 0.0445

MarkerSeek score 73.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 767
variable_sites: 34
pi: 0.0445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CTGCAGCTCATATTGGGGAA	45.0	50.0	58.1	57.6	835-838 bp	1.0	79.9
atpH-atpI_p2	ACAGAAGCGGCAGAAATCAA	CTGCAGCTCATATTGGGGAA	45.0	50.0	58.1	57.6	834-837 bp	1.0	79.9
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	CTTCGGCTGCAGCTCATATT	45.0	50.0	58.1	58.4	841-844 bp	1.0	79.4
atpH-atpI_p4	ACAGAAGCGGCAGAAATCAA	CTTCGGCTGCAGCTCATATT	45.0	50.0	58.1	58.4	840-843 bp	1.0	79.4
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	CTGCAGCTCATATTGGGGAA	50.0	50.0	57.8	57.6	845-848 bp	1.0	79.3