Hotspot

psbI-trnS-GCU

Genome · 7802-7869 · 68 bp

Back to Pentarhizidium

Pi 0.1

MarkerSeek score 81.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 68
variable_sites: 6
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TTTCTGTCGAATGATCCCGG	TTCGAATCCCTCTCTCTCCC	50.0	55.0	57.7	57.9	126-133 bp	1.0	84.6
psbI-trnS-GCU_p2	TTTCTGTCGAATGATCCCGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.7	58.2	129-136 bp	1.0	83.4
psbI-trnS-GCU_p3	TTCTGTCGAATGATCCCGGA	TTCGAATCCCTCTCTCTCCC	50.0	55.0	58.5	57.9	125-132 bp	1.0	83.0
psbI-trnS-GCU_p4	TTTCTGTCGAATGATCCCGG	GCATCCCTAGTTGTTCGGAG	50.0	55.0	57.7	58.1	210-217 bp	1.0	82.3
psbI-trnS-GCU_p5	TTCTGTCGAATGATCCCGGA	GCATCCCTAGTTGTTCGGAG	50.0	55.0	58.5	58.1	209-216 bp	1.0	80.6