Hotspot

psbK-psbI

Genome · 7174-7690 · 517 bp

Back to Pentarhizidium

Pi 0.0465

MarkerSeek score 71.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 517
variable_sites: 24
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCAGTTAGTTTTCG	CCGGGATCATTCGACAGAAA	50.0	50.0	58.0	57.7	626-627 bp	1.0	80.3
psbK-psbI_p2	CAAGCCGCAGTTAGTTTTCG	TCCGGGATCATTCGACAGAA	50.0	50.0	58.0	58.5	627-628 bp	1.0	78.6
psbK-psbI_p3	GCAAGCCGCAGTTAGTTTTC	CCGGGATCATTCGACAGAAA	50.0	50.0	58.3	57.7	627-628 bp	1.0	78.2
psbK-psbI_p4	CAAGCCGCAGTTAGTTTTCG	GGTTTCGTCCGGGATCATTC	50.0	55.0	58.0	58.7	634-635 bp	1.0	77.1
psbK-psbI_p5	GCAAGCCGCAGTTAGTTTTC	TCCGGGATCATTCGACAGAA	50.0	50.0	58.3	58.5	628-629 bp	1.0	76.5