Hotspot

ycf3

LSC · 41787-43738 · 1952 bp

Back to Pentanema

Pi 0.000342

MarkerSeek score 45.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1952
variable_sites: 1
pi: 0.000342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATGGGGCCAACAAACAAT	TCCCGCGAGAAGTAATGAGA	45.0	50.0	58.3	58.2	2088 bp	1.0	75.9
ycf3_p2	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCGCGAGAA	45.0	50.0	58.3	58.3	2097 bp	1.0	75.2
ycf3_p3	TGATGGGGCCAACAAACAAT	ATCATTTTCCCGCGAGAAGT	45.0	45.0	58.3	57.6	2095 bp	1.0	74.6
ycf3_p4	ATTTGACTGATGGGGCCAAC	TCCCGCGAGAAGTAATGAGA	50.0	50.0	58.4	58.2	2095 bp	1.0	74.6
ycf3_p5	ATTTGACTGATGGGGCCAAC	GGATCATTTTCCCGCGAGAA	50.0	50.0	58.4	58.3	2104 bp	1.0	73.8