Hotspot

ycf2

IRb · 84898-91746 · 6849 bp

Back to Pentanema

Pi 0.00311

MarkerSeek score 27.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6849
variable_sites: 32
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GAGATGTCAAAGGGGCTTCT	TGATAAATACTGATAACTCTCGGA	50.0	33.3	57.2	54.7	195 bp	1.0	46.2
ycf2_p2	AGAGATGTCAAAGGGGCTTC	TGATAAATACTGATAACTCTCGGA	50.0	33.3	57.2	54.7	196 bp	1.0	46.1
ycf2_p3	GAAGAGATGTCAAAGGGGCT	TGATAAATACTGATAACTCTCGGA	50.0	33.3	57.2	54.7	198 bp	1.0	46.1
ycf2_p4	TGAAGAGATGTCAAAGGGGC	TGATAAATACTGATAACTCTCGGA	50.0	33.3	57.5	54.7	199 bp	1.0	46.0
ycf2_p5	TGAAGAGATGTCAAAGGGGC	TTGATAAATACTGATAACTCTCGGA	50.0	32.0	57.5	55.4	200 bp	1.0	46.0