Hotspot

rpl32-trnL

SSC · 111145-112032 · 888 bp

Back to Pentanema

Pi 0.000751

MarkerSeek score 46.70

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 1
pi: 0.000751
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAAGGAAAGGGTATTGGGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1021 bp	1.0	78.8
rpl32-trnL_p2	AAAGGAAAGGGTATTGGGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1022 bp	1.0	78.8
rpl32-trnL_p3	GTATTGGGCGGCGTTAAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1011 bp	1.0	78.6
rpl32-trnL_p4	GTATTGGGCGGCGTTAAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	1012 bp	1.0	78.5
rpl32-trnL_p5	AAAGGAAAGGGTATTGGGCG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1031 bp	1.0	77.9