Hotspot

ycf2-trnL

IRb · 91747-92165 · 419 bp

Back to Pentanema

Pi 0.0207

MarkerSeek score 47.80

Primer available yes

Label ycf2-trnL

Feature Metadata

label_name: ycf2-trnL
feature_type: igs
region: IRb
length_bp: 419
variable_sites: 13
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL_p1	CCGGAAAGATATGTGGCCAT	AAATGGTAGACACGCGAGAC	50.0	50.0	57.7	58.0	510-994 bp	1.0	83.3
ycf2-trnL_p2	CCGGAAAGATATGTGGCCAT	TGAAATGGTAGACACGCGAG	50.0	50.0	57.7	58.0	512-996 bp	1.0	83.3
ycf2-trnL_p3	CCCATTCCTTAGCCGGAAAG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.3	58.0	522-1006 bp	1.0	82.9
ycf2-trnL_p4	CCCATTCCTTAGCCGGAAAG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.3	58.0	524-1008 bp	1.0	82.9
ycf2-trnL_p5	CCGGAAAGATATGTGGCCAT	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.7	58.1	522-1006 bp	1.0	82.7