Hotspot

rps16-trnQ-UUG

LSC · 6278-7179 · 902 bp

Back to Pentanema

Pi 0.000741
MarkerSeek score 46.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
902
variable_sites
1
pi
0.000741
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 973-975 bp 1.0 78.5
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 984-986 bp 1.0 78.0
rps16-trnQ-UUG_p3 CACACCGGATCATGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 993-995 bp 1.0 76.9
rps16-trnQ-UUG_p4 CACCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 991-993 bp 1.0 76.4
rps16-trnQ-UUG_p5 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1044-1046 bp 1.0 75.6