Hotspot

ycf1

SSC · 125680-131319 · 5640 bp

Back to Pentactina

Pi 0.00642

MarkerSeek score 47.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 36
pi: 0.00642
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATCCTCTGAATCAATCCT	AGGGTTTGGATAAATAAGATTCATGG	42.9	34.6	55.9	57.5	222 bp	1.0	42.2
ycf1_p2	TCGATCCTCTGAATCAATCCT	GGGTTTGGATAAATAAGATTCATGGT	42.9	34.6	55.9	57.7	221 bp	1.0	41.8
ycf1_p3	TCGATCCTCTGAATCAATCCT	ACATAGAAATAGAAAGGGTTTGGA	42.9	33.3	55.9	55.8	236 bp	0.5	20.0
ycf1_p4	TCGATCCTCTGAATCAATCCTT	ACATAGAAATAGAAAGGGTTTGGA	40.9	33.3	56.7	55.8	236 bp	0.5	20.0
ycf1_p5	TTCGATCCTCTGAATCAATCCT	ACATAGAAATAGAAAGGGTTTGGA	40.9	33.3	56.7	55.8	237 bp	0.5	20.0